Guidelines
Blood Sample
Following steps are crucial for ensuring accurate and safe blood collection for diagnostic purposes. Each step plays a vital role in the process, from verifying patient identity to proper handling of the collected sample. Staff MUST wear gloves while performing following procedures. Here's a brief overview of each step:
1. Verify patient identity and fasting requirements: Confirming the patient's identity helps ensure that the sample is correctly labeled and attributed. Additionally, fasting requirements may be necessary for certain tests to obtain accurate results.
2. Label the sample tube: Proper labeling of the sample tube with patient full name,age, gender, date and time of collection is essential for tracking and maintaining the integrity of the sample. If multiple samples are collected, ensure each is properly labeled.
3. Clean the collection site of patient arm: Cleaning the collection site with an alcohol swab helps reduce the risk of contamination, minimizing the chances of introducing external substances into the sample.
4. Apply a tourniquet: Applying a tourniquet helps locate and dilate the vein, making it easier to insert the needle and collect the required blood volume.
5. Insert a sterile needle / vaccutainer: Using a sterile needle, insert it into the vein to collect the necessary amount of blood for testing in appropriate tube.
6. Invert the sample tube: Gently invert the sample tube to mix the collected blood with any additives present in the tube. This ensures proper mixing and distribution of the sample components.
7. Remove the tourniquet and needle / vaccutainer, and apply pressure: After collecting the blood sample, remove the tourniquet and needle and apply pressure to the puncture site to prevent bleeding and promote clotting.
Urine 24 hr
Collecting a 24 Hr Urine sample for medical analysis typically involves the following steps:
1. Verify patient identity and fasting requirements: Confirming the patient's identity helps ensure that the sample is correctly labeled and attributed. Additionally, fasting requirements may be necessary for certain tests to obtain accurate results.
2. Preparation: Ensure all materials are clean and sterile. Wash hands thoroughly with soap and water. Prepare the urine collection containers by labeling them with the patient's name, date, and time of collection.
3. Instructions for the Patient: Inform the patient about the importance of collecting all urine passed over the 24-hour period. Advise the patient to begin the collection process in the morning upon waking. Instruct the patient to discard the first urine passed upon waking and note the time. All subsequent urine passed over the next 24 hours should be collected in the provided container.
4. Collection: Provide the patient with the urine collection hat or container to use when voiding. Ensure the patient understands the importance of collecting all urine, including overnight specimens. Instruct the patient to store the collection container in a cool place, preferably the refrigerator, between voids. Advise the patient to avoid contact with chemicals or contaminants during collection.
5. Monitoring: Check in with the patient periodically to ensure compliance and address any concerns. Remind the patient to record any medications taken during the collection period.
6. Completion: After 24 hours have elapsed, instruct the patient to empty their bladder one final time and add this urine to the collection container. Seal the collection container tightly to prevent leakage. Record the total volume of urine collected on the container label. Place the container in the refrigerator for storage until it can be transported to the laboratory.
7. Transportation to the Laboratory: Arrange for the timely transport of the urine sample to the laboratory, following appropriate handling and transportation procedures. Ensure the sample remains refrigerated during transit to maintain sample integrity.
Notes: It is essential to ensure patient compliance and proper collection techniques to obtain accurate results. Any deviations from the standard procedure should be documented and reported accordingly. Laboratory processing protocols may vary, so ensure compliance with specific laboratory requirements.
Stool / Stool Culture
Collecting a stool sample for medical analysis typically involves the following steps:
1. Verify patient identity: Confirming the patient's identity helps ensure that the stool sample is correctly labeled and attributed.
2. Preparation: Wash your hands thoroughly with soap and warm water before starting.
3. Avoid Contamination: Ensure that urine and toilet paper do not come into contact with the stool sample, as they can affect the results.
4. Collect the Sample: Use a clean, dry, sterile container with a tight-fitting lid. Ensure not to touch the inside of the container or the stool with your hands.
5. Clean Up: Dispose of any materials used for collection properly. Wash your hands thoroughly with soap and water again after handling the sample.
6. Storage and Transport: Store the sample as per the instructions provided by your healthcare provider. This may involve refrigeration or keeping it at room temperature, depending on the specific tests being performed. If you need to transport the sample to a healthcare facility, do so promptly, following any instructions regarding transportation and handling.
Remember, it's crucial to follow any specific instructions provided by your healthcare provider for collecting and handling the stool sample, as requirements may vary depending on the tests being conducted.
Urine Routine / Urine Culture
Collecting a urine sample for routine analysis typically involves the following steps:
1. Verify patient identity: Confirming the patient's identity helps ensure that the urine sample is correctly labeled and attributed.
2. Preparation: Wash your hands thoroughly with soap and warm water before starting. Obtain a clean, dry and sterile container for collecting the urine sample.
3. Avoid Contamination: Ensure that the container and its lid remain clean and do not come into contact with any surfaces, including your hands, to prevent contamination.
4. Midstream Collection (for clean-catch urine sample): If instructed by your healthcare provider, clean your genital area with a sterile wipe or tissue. Start urinating into the toilet to clear the urethra of any contaminants. Without interrupting the flow of urine, position the container under the urine stream to collect a midstream. Avoid collecting start and last 5-8 drops of urine. Collect a sufficient amount of urine, usually about halfway through urination, as specified by your healthcare provider. Avoid touching the inside of the container or the rim with your hands or body.
5. Secure the Lid: Immediately after collection, securely close the lid of the container to prevent leakage or contamination.
6. Clean Up: Dispose of any materials used for collection properly. Wash your hands thoroughly with soap and water again after handling the sample.
7. Storage and Transport: If you need to transport the sample to a healthcare facility, do so promptly, following any instructions regarding transportation and handling. In most cases, you should deliver the sample within a specified timeframe to ensure the accuracy of the results.
Always follow any specific instructions provided by your healthcare provider for collecting and handling the urine sample, as requirements may vary depending on the specific tests being conducted.
Pus
Staff MUST wear gloves while performing following procedures. Collecting a pus sample for medical analysis typically involves the following steps:
1. Verify patient identity: Confirming the patient's identity helps ensure that the pus sample is correctly labeled and attributed.
2. Sterile Technique: Ensure that all materials and surfaces remain sterile throughout the procedure to avoid introducing contaminants into the sample.
3. Cleaning the Area: If the pus is located in an external wound or abscess, clean the surrounding area with an antiseptic solution and allow it to dry. Avoid touching the area with your hands after cleaning.
4. Collection Method: Depending on the location and nature of the pus, there are different collection methods: *Swab Collection: Using a sterile swab, gently rub the swab over the surface of the pus, ensuring good contact to collect the sample. Carefully place the swab into the sterile container without touching the surrounding area. *Aspiration: If the pus is within a deeper wound or abscess, a healthcare provider may use a needle and syringe to aspirate (draw out) the pus. This procedure should only be performed by trained personnel.
5. Sample Transfer: Ensure that the collected pus is transferred into the sterile container without contamination. For swab samples, break or cut the swab stick, leaving the swab tip inside the container. If aspiration was performed, transfer the pus from the syringe into the sterile container.
6. Sealing the Container: Securely close the lid of the sterile container to prevent leakage or contamination.
7. Clean Up: Dispose of any materials used for collection properly, following appropriate biohazard waste disposal protocols if applicable. Remove and dispose of sterile gloves properly. Wash your hands thoroughly with soap and water after handling the sample.
8. Storage and Transport: Store the sample as per any specific instructions provided by your healthcare provider or laboratory. This may involve refrigeration or keeping it at room temperature. If the sample needs to be transported to a healthcare facility or laboratory, follow any instructions regarding transportation and handling to maintain sample integrity.
Always follow any specific instructions provided by your healthcare provider or laboratory for collecting and handling pus samples to ensure accurate testing and diagnosis.
Histopath
(FFPE block) Tumor containing tissue fixed in 10% neutral buffered formalin with duration of 6 to 72 hours and paraffin embedded. Clinical history with Histopath/IHC report along with doctors number. FFPE block to be shipped at Ambient temperature.
TB sample Sputum
Staff MUST wear gloves and mask while performing following procedures. Collecting a sptum sample for medical analysis typically involves the following steps:
1. Verify patient identity: Confirming the patient's identity helps ensure that the sputum sample is correctly labeled and attributed.
2. Instruct patient: Patient must rinse their mouth with water to reduce contamination.
3. Guidelines: Ask the patient to take a deep breath and cough deeply to produce sputum from the lower respiratory tract.
4. Sample Collection: Collect the expectorated sputum directly into a sterile container.
5. Precaution: Ensure that the sputum sample is representative and free of saliva contamination. Tightly seal the container
6. Transport: Send the sample to the laboratory promptly for analysis. Staff must collect patient history, doctor prescription if any, Adhar number, address, email address, alternative contact details. Report must infrom by lab to BMC or govt. authority.
TB Any sample
Staff MUST wear gloves and mask while performing following procedures. Staff must collect patient history, doctor prescription if any, Adhar number, address, email address, alternative contact details. Report must infrom by lab to BMC or govt. authority.
Glucose test
For the 75mg glucose test, follow procedure:
1. A lab technician will take a sample of blood from your vein. They will test this fasting blood sugar for the baseline amount of sugar in your blood.
2. After this, you will be given a glucose solution to drink. Most of such solutions contain 75 grams of glucose. (75 gm of glucose need to dissolve in 250 to 300 ml of water). You are advised to sit down or lay for about an hour.
3. After 1 hour, the technician takes another blood sample for testing. They may take more blood samples after 2 hours and after 3 hours after taking the glucose solution.
4. Between testing times, it is advised to the patient to not be too active and not to drink too much water. This is because excess movement and hydration can impact the concentration of glucose in the blood. It can in turn adversely affect the test results.